New York, USA – February 25, 2021 – Profacgen, a state-of-the-art protein service provider located in NY, USA, providing custom protein services in the biological sciences, now offers various services for PROTAC In Vitro Evaluation.
In vitro (cell-based and non-cell-based assays) assessment of candidate PROTAC is often based on biological activity against target proteins, which helps to identify binding affinity, permeability, degradation and stability, cytotoxicity, and others. In in vitro tests, different kinds of cells and tissues (possibly from humans, animals, microorganisms and even plants) can provide evidence about changes in the activity and function of target proteins caused by PROTAC.
Now, Profacgen provides various in vitro evaluation services for its customers’ projects with professional scientist team.
Enzyme leakage assays, Membrane impermeable dyes, Amine-reactive dyes, Dye combination assays, Apoptosis assays, Cell proliferation and cell cycle assays, Cell viability assays
Degradation Assay and Stability Analysis
Antibody based Western blot or ELISA assay, Mass spectrometry (MS), Real-time degradation kinetics measurement, Enzyme Fragment Complementation (EFC) technology
Western blot-based E3 ligase activity assay, Enzyme-Linked Immunosorbent Assay (ELISA)-based E3 ligase activity assay, Tandem Ubiquitin Binding Entities (TUBEs)-based E3 ligase activity assay, Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)-based E3 ligase activity assay
Parallel Artificial Membrane Permeability Assay (PAMPA), Caco-2 Permeability Assay, Madin – Darby canine kidney cell line (MDCK) Permeability Assay
Ternary Complex Formation
Isothermal titration calorimetry (ITC), Dynamic light scattering (DLS), Fluorescence polarization (FP), X-ray crystallography, Fluorescence Resonance Energy Transfer (FRET), Cell-free Proximity Assays, Bioluminescence Resonance Energy Transfer (BRET)
Immunoprecipitate (IP) and Western blot, Enzyme-linked Immunosorbent Assay (ELISA), Mass Spectrometry (MS)
“Supported by one-stop platform and experienced technical team, high data quality and reliable analysis, cost-effective pricing and short turnaround times, we are confident to help accelerate our customers’ research progress.” Commented Crystal, the chief marketing staff at Profacgen.
If you feel interested and want to know more, please visit https://www.profacgen.com.
Profacgen provides process development and cGMP manufacturing services for biological active ingredients. We have ready access to state-of-the-art biopharmaceutical product development and manufacturing facilities and our staff scientists have demonstrable expertise in all aspects of biopharmaceutical manufacturing, from facility design and operation to the ability to provide best advice relating to product development.