New York, USA – February 5, 2021 – Membrane proteins on the cell surface stimulate various biological pathways and regulate important cellular functions, such as cell interaction, material transport, energy conversion, information reception, recognition, and transmission. Many abnormal expressions of membrane proteins were reported to be disease-related or as targets for drug treatment. Taking cancer as an example, quite a ratio of the cancer biomarkers used in clinical practice today are membrane proteins. Creative Biolabs, as a professional service provider of new drug development, is working devotedly on this powerful tool for disease treatment and has successfully established the Magic™ platform specialized for membrane protein production, with the aim of improving the research in relevant fields.
The powerful platform fulfills multiple functions, including expression, solubilization, reconstitution, and characterization, and provides various options of host organisms such as E.coli, yeast cells, and mammalian cells.
* Cell-based membrane protein expression-yield the membrane proteins of interest from recombinant genes in living cell system.
* Cell-free membrane protein expression-superior to conventional cell-based systems, and based on the modified lysates of various cellular components, which are classified as prokaryotic system, eukaryotic system, and PURE system.
* Virus-like particles (VLPs) and lipoparticles-HBV VLP, HIV VLP, HPV VLP, CA16 VLP, EV71 VLP, poliovirus VLP, MERS-CoV VLP, SARS-CoV VLP, SARS-CoV-2 VLP, etc.
The various systems embedded in the Magic™ platform apply to the development of membrane proteins with various functions, including anti-membrane protein antibody and membrane transport protein.
Solubilization of membrane proteins is one of the technical difficulties. Creative Biolabs has constructed membrane protein overexpressing stable cell lines that are applicable for membrane isolation, immunization, and assays like SPR, allowing for membrane proteins to be reconstituted in detergents (micelles), liposomes, nanodiscs (lipids, membrane scaffolding proteins), and polymers.
Years of hands-on experience and high-end technology platform contribute to the successful development of G-protein coupled receptors (GPCRs), ion channels, membrane transport proteins, etc., which are difficult to obtain. Several membrane protein products with multiple species and tags are readily available for quick global delivery, which have high purity and low endotoxin. More details about the platform and the products can be found at https://www.creative-biolabs.com.
Creative Biolabs is a biotechnology company that is specialized in antibody & protein production and engineering, providing customized solutions to global clients as a support of their projects related to disease diagnosis and therapies. For over ten years of exploration and expansion, it has developed into an international enterprise that is based in the US and has offices in European countries.